For purification of total dna from animal blood, animal tissue, rodent tails, ear punches, cultured cells, fixed tissue, bacteria, insects. Qiagen plasmid purification handbook qiagen plasmid midi, maxi, mega, and giga kits qiafilter plasmid midi, maxi, mega, and giga kits endofree plasmid maxi, mega, and giga kits for purification of ultrapure plasmid dna second edition august 2003. Qiagen plasmid midi kit, 100 preps, 25 to 100ml culture volume, manual centrifugation processing, 150 min. The qiaamp minelute virus spin kit simplifies purification of viral dna and rna with fast spincolumn procedures. The rneasy midi kit provides fast purification of highquality rna from cells, tissues, and yeast using silicamembrane rneasy spin columns with a binding capacity of 1 mg rna. The kit uses a rapid purification procedure based on the remarkable. Plasmid kits, or qiagen products in general, please do not hesitate to contact us. Our technical service departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of qiagen prod ucts. Qiagen plasmid kits provide gravityflow, anionexchange tips for purification of transfectiongrade plasmid dna.
Do not add bleach or acidic solutions directly to the samplepreparation waste. Qiaamp dna blood midi kit 100 for 100 dna midipreps. This information is helpful to other scientists as well as to. The design and unique binding chemistry of the qiagen plasmid plus columns allow a simple bindwashelute procedure based on a novel chemistry. Anionexchangebased qiagentips yield transfection grade dna, which is highly. Qiaprep miniprep handbook university of pittsburgh. Plasmid or cosmid dna purification using qiagen plasmid midi and maxi kits. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food and drug. The qiaamp dna blood midi kit processes sample sizes of 0. Hispeed plasmid purification handbook harvard university. Rneasy lipid tissue kits or qiagen products in general, please do not hesitate to.
Rneasy lipid tissue handbook 022009 3 contents kit contents 4 storage 4. For purification of up to 100ug transfection grade plasmid or cosmid dna. Lysate clearing and isopropanol precipitation are achieved by centrifugation. Filmed at the university of northampton, by adam gregory. Qia announced today that it has received clearance for its ipsogen jak2 rgq pcr kit ipsogen. Qiaquick spin kits are developed, designed and sold for research purposes only.
The kit contains optimized components that allow both reverse transcription and pcr amplification to take place in what is commonly referred to as a onestep reaction. The qiaamp minelute virus spin kit uses starting sample volumes of up to 0. Qiagen protease provided in the qiaamp dna blood midi kit 20, or 5. Both the qiaprep spin miniprep kits and qiagen plasmid plus kits work with the manifold, which can handle up to 24 samples at the. For the production of printed materials, including handbooks, qiagen has a policy to select suppliers that comply with fsc standards for printing processes and wellmanaged forests. This procedure makes these kits the fastest largescale prep available 20 minutes for maxi and midi preps. Qiagen plasmid plus midi kit 25 from qiagen selectscience. Midiprep plasmid dna extraction kit is used when the starting e. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food and drug administration in the usa. Hispeed plasmid midi and maxi kits are developed, designed, and sold for research. Qiagen plasmid purification handbook kirschner lab. Qiagen customers are also a major source of information regarding advanced or specialized uses of our products.
The qiagen plasmid plus kits enable ultrafast, largescale purification of up to 10 mg of highly pure plasmid dna. Do not add bleach or acidic solutions directly to the samplepreparation. Hispeed plasmid purification handbook kirschner lab. Full qiagen maxiprep plasmid dna extraction with steps. Qiasymphony dsp viruspathogen kit instructions for use. Qiagen clinical kits compatible with the qiavac 24 plus application kit accessories genomic and viral dna purification from blood and body fluids qiaamp dna blood kits vacconnectors and vacvalves qiaamp dna mini kit vacconnectors and vacvalves viral rna purification from plasma. A variety of microorganisms, including bacterial and fungal spores, have been tested successfully with this kit. Rneasy mini kit for purification of total rna from animal cells, animal tissues, and yeast, and for cleanup of rna from crude rna preps and enzymatic reactions e. Rneasy minelute cleanup handbook 032003 5 product warranty and satisfaction guarantee qiagen guarantees the performance of all products in the manner described in our product literature. Plasmid purification using a qiagen hispeed plasmid maxi kit. Qiagen plasmid mini, midi, maxi, mega, and giga kits. Qiagen s position as the market leader for sample and assay technologies is based on more than 25 years of experience, strong relationships with customers, o. The following risk and safety phrases apply to components of the rneasy mini kit, the rneasy protect mini kit, andor the rneasy plant mini kit.
Filmed using an iphone xsmax and inbuilt imovie features. Handbook for hispeed plasmid midi kit hispeed plasmid maxi kit. Oncology solutions molecular diagnostics and research. Timerun, up to 1mg yield, ideal transfection of most cell lines, cloning. Qiagen plasmid plus kits provide a novel patentpending method for extremely fast and easy largescale preparation of transfectongrade plasmid dna. The kit uses qiaamp minelute spin columns for purification of highquality dna with flexible elution volumes. Introduction qiagen pcr cloning kits combine the latest ligation technology with a unique combination of timesaving features for fast, easy, and highly efficient cloning of pcr products generated. Qiaamp dna blood kits are supplied with a comprehensive handbook containing protocols for dna isolation from a number of sample sources. Refer to the troubleshooting guide in the relevant handbook view faqs contact technical service. For highthroughput purification in a 96well format, the mirneasy 96 kit is available. Qiagen plasmid purification handbook 032020 3 kit contents provided in a 10 mgml or 100 mgml solution. Qiagen is a member of the forest stewardship council fsc.
The purchaser must determine the suitability of the product for its particular use. Qiagen plasmid plus technology delivers the same performance and quality as anionexchange technology. The qiagen largeconstruct kit protocol starts with modified alkaline lysis and integrated. The qiagen plasmid plus kits provide the most convenient method for largescale plasmid preparation.
Rneasy minelute cleanup handbook harvard university. Under these conditions, minelute kits can be stored for up to 12 months without showing any reduction in performance and quality. Qiaprep spin midimaxiprep kit protocol before staring make sure that rnase a has been added to p1 buffer. Qiaamp dna mini and blood mini handbook 112007 5 qiaamp dna kits blood mini 50 blood mini 250 mini 50 mini 250 catalog no. Qiagen plasmid purification handbook sierra bermeja. The procedure can be performed in 20 midi and maxi, 40 mega, or 50 minutes giga using a. Total rna and mirna can also be copurified from formalinfixed, paraffinembedded. Qiagen distributors please see the last page for contact information for your local qiagen distributor. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. The rneasy mini handbook provides protocols for use with the following kits. Should any product fail to perform satisfactorily due to any reason other. Qiagen plasmid purification handbook harvard university. Qiagen plasmid plus midi kit, 25 preps, 25 to 50ml culture volume, transfectiongrade plasmid or cosmid dna purification, advanced silica technology, manual processing, 20 min.
Low elution volumes yield highly concentrated plasmid dna for direct use without ethanol precipitation. Qiagen plasmid midi kit, 25 preps, 25 to 100ml culture volume, manual centrifugation processing, 150 min. Troubleshooting molecular biology applications qiagen. For the fastest, most convenient purification of up to 10 mg transfectiongrade plasmid dna. Full plasmid dna extraction completed using a qiagen maxiprep kit. The qiaamp dna investigator kit provides purification of genomic dna from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. If you need support with our regulated products, contact your local technical service department directly. For purification of ultrapure, transfectiongrade plasmid dna. For largescale genomic and viral dna purification from whole blood, plasma, serum, body fluids, lymphocytes. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing handson time. For purification of ultrapure, transfection grade plasmid dna.
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